LOCUS       sequence-110676-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (TaU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN421
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    104..278
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 tgtactgaat taacgccgaa ttgagttaac ctgactagtg acaacttaat aacacattgc
       61 ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac
      121 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
      181 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
      241 ccttatctgg gaactactca cacattatta tggagaaact cgagtcaaat ctcggtgacg
      301 ggcaggaccg gacggggcgg taccggcagg ctgaagtcca gctgccagaa acccacgtca
      361 tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc cgcggggggc atatccgagc
      421 gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga tgacagcgac cacgctcttg
      481 aagccctgtg cctccaggga cttcagcagg tgggtgtaga gcgtggagcc cagtcccgtc
      541 cgctggtggc ggggggagac gtacacggtc gactcggccg tccagtcgta ggcgttgcgt
      601 gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc cgtccacctc ggcgacgagc
      661 cagggatagc gctcccgcag acggacgagg tcgtccgtcc actcctgcgg ttcctgcggc
      721 tcggtacgga agttgaccgt gcttgtctcg atgtagtggt tgacgatggt gcagaccgcc
      781 ggcatgtccg cctcggtggc
//
