LOCUS       sequence-110685-         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Measurements of translational accuracy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   O'Connor M, Dahlberg AE
  TITLE     Mutations at U2555, a tRNA-protected base in 23S rRNA, affect 
            translational fidelity.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8.
  PUBMED    8415679
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-10-1"; volume: "90(19)"; pages: 
            "9214-8"
COMMENT     Sequence Label: pSG 12-6
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..162
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     154..171
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     155..171
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(224..246)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    235..251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(259..265,266..281,282..287))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
       61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattaggg
      181 ccggcctaaa caaagcttag cgtaatcatg gtgaattctg tttcctgtgt gaaattgtta
      241 tccgctcaca attgtcgaca ttatacgagc cgatgattaa ttgtcaacac ggccgacgcg
      301 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
      361 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
      421 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
      481 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
      541 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
      601 gcatggagcc gggccacctc gacctgaatg gaagccactg c
//
