LOCUS       sequence-110688-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: beta-catenin (S33Y)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg caacaggtca
      121 gtatcaaacc aggccagctg attgctgtca cctggaggca gcccatccat gaggtcctgg
      181 gcatgcccca gatctggcag cccatcaact ggatagtcag caccagggtg gtggccaccc
      241 atctcatgtt ccatcatggg gtccataccc aaggcatcct ggccatatcc accagagtga
      301 aaagaacgat agctaggatc atcctggcga tatccaaggg gttctccctg ggcaccaata
      361 tcaagtccaa gatcagcagt ctcattccaa gccattggct ctgttctgaa gagagagctg
      421 gtcagctcaa ctgaaagccg tttcttgtaa tcttgtggct tgtcctcaga cattcggaac
      481 aaaacagcag ctgcatatgt cgccacacct tcattcctag agtgaagtaa ctctgtcaga
      541 ggagctgtgg ctccctcagc ttcaatagct tctgcagctt ccttgtcctg agcaagttca
      601 cagaggaccc ctgcagctac tctttggatg ttttcaatgg gagaataaag cagctgcaca
      661 aacaatggaa tggtatttag tcctctgata acaattcggt tgtgaacatc ccgagcta
//
