LOCUS       sequence-110694-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying a modified HSVtk gene as a 
            negative selection marker against ectopic transformants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    53..227
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     444..466
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     458..475
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     459..475
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     485..504
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        485..503
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     528..544
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     529..545
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(579..595)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(579..595)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga
       61 ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa
      121 atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa
      181 ccctaattcc cttatctggg aactactcac acattattat ggagaaactc gattggcaag
      241 ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaaa
      301 cattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
      361 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
      421 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
      481 cgcgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg
      541 tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc
      601 cgccaccgcg gtgaattcga attgggtact caaattggtt cccgcttgac gacattccga
      661 aacccccaat ttcgcggctt cgaatcgtgg ctacccttgg ccgaggttgc gatttctctg
      721 ccgtatctga caatgatcca accggccgga tgtgtgggag ggcgcaaagg gtgtaattgg
      781 gtctaggcgt gagctgcatg tccgatggca gaaaaagaac tactaaaact ctgtgacgtc
      841 ctcagggctg gtagctattt agcttgcccc tccctcaccc cac
//
