LOCUS       sequence-110699-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: MEK5 DD(S311D,T315D)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg caacgggggc
      121 ccctgctggc tccgcctctc ctccagcgcc cggcacaccc acatggacac cacggcggca
      181 tttccatcat tgaactgcac gatgaacggg tggcccatca attcttcagg tgctggcctt
      241 tcttttggct gttttcgcat acactgagtg atgaaatgta caaatggctc cgagaactct
      301 ccaattggaa ggacgggcga atcctcatca acaatgcact gcagaagctg gagaggcatt
      361 aaagatccct ggtttttctg aatctgagga tatggaaacc tcccaagagc aagctccata
      421 aaagagattc ctaagctcca gacatcagaa tgaattccat actgctcccc tgaaatcctt
      481 tcaggcgcca tataagcatt tgttccaaca taatccttgg ctatatcatt caccagctga
      541 gtgctaactc caaaatcaca cagcttaacc tgtcctcttg tgtttactag catattggag
      601 ggcttcacgt ctctatgtaa aatctttaaa ctccacaaat aagtaaggcc tttaacaact
      661 gctactgcaa ttcttccaag gacatgttct ggcattttcc tatatacatc caaagat
//
