LOCUS       sequence-110700-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-MEK5-pcw107
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             83..124
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             149..172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    179..203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaccgacct ctctccccag gggctagcat cgatggatca acaagtttgt acaaaaaagt
       61 tggcacaggc ggcggcggca ccatggggtc ttcaaaatct aaaccaaagg accccagcca
      121 gcgccggcgc aggatccgag gttaccttga ctacaaagac gatgacgaca agcaattgac
      181 aagtttgtac aaaaaagttg gcatgctgtg gctagccctt ggcccctttc ctgccatgga
      241 gaaccaggtg ctggtaattc gcatcaagat cccaaatagt ggcgcggtgg actggacagt
      301 gcactccggg ccgcagttac tcttcaggga tgtgctggat gtgataggcc aggttctgcc
      361 tgaagcaaca actacagcat ttgaatatga agatgaagat ggtgatcgaa ttacagtgag
      421 aagtgatgag gaaatgaagg caatgctgtc atattattat tccacagtaa tggaacagca
      481 agtaaatgga cagttaatag agcctctgca gatatttcca agagcctgca agcctcctgg
      541 ggaacggaac atacatggcc tgaaggtgaa tactcgggcc ggaccctctc aacacagcag
      601 cccagcagtc tcagattcac ttccaagcaa tagcttaaag aagtcttctg ctgaactgaa
      661 aaaaatacta gccaatggcc agatgaatga acaagacata cgatatcggg acactcttgg
      721 tcatggcaac ggaggcacag tctac
//
