LOCUS       sequence-110701-        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli expression plasmid to produce Thrombin cleavable His tagged 
            iLOV fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  TITLE     B Smith Lab iLOF fusion plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-iLOVf
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(174..629)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(311..332)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     523..542
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(666..713)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     709..727
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(780..797)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(846..878)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(882..899)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(909..926)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
       61 gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt
      121 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
      181 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
      241 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
      301 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
      361 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
      421 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
      481 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
      541 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
      601 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccggatat agttcctcct
      661 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc
      721 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatctcag
      781 tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt cgacggagct cgaattcgga
      841 tcccgaccca tttgctgtcc accagtcatg ctagccatat ggctgccgcg cggcaccagg
      901 ccgctgctgt gatgatgatg atgatggctg ctgcccatgg aatgtacgtg gtcagaacca
      961 tccagttgaa cgccgatgaa atactgcagc tcgcctttct gatcgcggac tggttgcaga
     1021 tgcagcaggt tccagaattt cttaccgctt ttcgtgtagt taatcagttg caccgtagtt
     1081 tcacgctggt cacggatagc atcgcgaatt t
//
