LOCUS       sequence-110702-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-MEK5-pcw107
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg caatcacggg
      121 ggcccctgct ggctccgcct ctcctccagc gcccggcaca cccacatgga caccacggcg
      181 gcatttccat cattgaactg cacgatgaac gggtggccca tcaattcttc aggtgctggc
      241 ctttcttttg gctgttttcg catacactga gtgatgaaat gtacaaatgg ctccgagaac
      301 tctccaactg gaaggacggg cgaatcctca tcaacaatgc actgcagaag ctggagaggc
      361 attaaagatc cctggttttt ctgaatctga ggatatggaa acctcccaag agcaagctcc
      421 ataaaagaga ttcctaagct ccagacatca gaatgaattc catactgctc ccctgaaatc
      481 ctttcaggcg ccatataagc atttgttcca acatacgtct tggctataga attcaccagc
      541 tgagtgctaa ctccaaaatc acacagctta acctgtcctc ttgtgtttac tagcatattg
      601 gagggcttca cgtctctatg taaaatcttt aaactccaca aataagtaag gcctttaaca
      661 actgctactg caattcttcc aaggacatgt tctggcattt tcctatatac atccaaagat
      721 cccccatcca tgaattctgt acatattg
//
