LOCUS       sequence-110706-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Notch1 intracellular domain-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..84)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cggtcattac tacgtagaat cgagaccgag
       61 gagagggtta gggataggct taccaccggt aaccactttg tacaagaaag ttggcaactt
      121 gaaggcctcc ggaatgcggg cgatctggga ctgcatgctg gtgggagggc tggagacgcc
      181 ctcggaccag tcggagacgt tggaatgcgg ggacgagctg gaccactggt caggggactc
      241 aggggacggg gtgaggaagg ggtgctcagg cacctgtagc tggtggctgg gggtgttgtc
      301 cacaggcgag gagtagctgt gctgcgaggg gggcgtcagg aactgggctg cggtcacggg
      361 tgggaccagc gaggatggca gcgacgtggg cagggcgggg ctctcctggg gcagaatagt
      421 gtgcaccgcc aggctgctgg ggcccagtgg ctgcacgtct gcctggctcg gctctccact
      481 caggaagctc cggcccaggt ggccgctggc tgctgagctc acgccaaggt gcggctgtgg
      541 tggtggtggt ggcggctgca ggctttgctg ctgctggatg tttgctggct gcaggttctg
      601 ctgctgcatc tgtaagtttt gtggctgcac ctgctgggtc tgcaccaggt gaggctgggt
      661 ggccagccgg gtgctgggca ggccctggta gctcatcatc tgggaca
//
