LOCUS       sequence-110709-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of Centrin-mCherry fusion and Puro resistance
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRES Centrin1 Cherry Puro
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      126..135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             132..839
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(277..295)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     545..564
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tctctggcta ctagaagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatcgatatc tgcggcctag ctagcgctac
      121 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      181 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      241 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      301 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      361 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      421 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      481 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      541 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      601 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      661 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      721 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      781 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      841 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc atggcttccg
      901 gcttcaagaa gcccagcgct gcctccaccg gccaaaaga
//
