LOCUS       sequence-110711-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: p38 WT O/E (MAPK14)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..81)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             195..203
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tccagagggt tgattgtcga cttaacgcgg tcattactac gtagaatcga gaccgaggag
       61 agggttaggg ataggcttac caccggtaac cactttgtac aagaaagttg gcaaggactc
      121 catctcttct tggtcaaggg gtggtggcac aaagctgatg acttcatcat aggtcaggct
      181 tttccactca tctataagga ggtccctgct ttcaaaggac tgatcataag gatcggccac
      241 tggttcatca tcaggatcgt ggtactgagc aaagtaggca tgtgcaaggg cttgggccgc
      301 tgtaattctc ttatctgagt ccaatacaag catcttctcc agcaagtcga cagccagggg
      361 attggcacca ataaatacat tcgcaaagtt catcttcggc atctgagtca aagactgaat
      421 atagtttctt gcagactctg aggagatttt cttcaaaagc tcagcccctg gggttccaac
      481 gagtcttaaa atgagcttca actgatcaat atggtctgta ccaggaaaca atgttcttcc
      541 agtcaacagc tcggccatta tgcatcccac tgaccaaata tcaactgtct ggttgtaatg
      601 catccagttc agcatgatct caggagccct gtaccaccta gtggccacgt agcctgtcat
      661 ttcatcatct gtgtgccgag ccagtccaaa atccagaatc ttcagctcac agtcttcatt
      721 cacagctaga ttacta
//
