LOCUS       sequence-110713-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of beta-galactosidase at high levels in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   O'Connor M, Dahlberg AE
  TITLE     Mutations at U2555, a tRNA-protected base in 23S rRNA, affect 
            translational fidelity.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8.
  PUBMED    8415679
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-10-1"; volume: "90(19)"; pages: 
            "9214-8"
COMMENT     Sequence Label: pSG 25
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..162
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     154..171
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     155..171
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(206..228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    217..233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(241..247,248..263,264..269))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
       61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
      181 agcgtaatca tggtgaattc tgtttcctgt gtgaaattgt tatccgctca caattgtcga
      241 cattatacga gccgatgatt aattgtcaac acggccgacg cgctgggcta cgtcttgctg
      301 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
      361 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
      421 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc
      481 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
      541 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
      601 tcgacctgaa tggaagccac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
      661 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
      721 atggca
//
