LOCUS       sequence-110715-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: FLAG-MKK6 (S207E,T211E)-pcw107
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gacctctctc cccaggggct agcatcgatg gatcaacaag tttgtacaaa aaagttggca
       61 acagcggcgg cggcaccatg gactacaaag acgatgatga caaatctcag tcgaaaggca
      121 agaagcgaaa ccctggcctt aaaattccaa aagaagcatt tgaacaacct cagaccagtt
      181 ccacaccacc tagagattta gactccaagg cttgcatttc tattggaaat cagaactttg
      241 aggtgaaggc agatgacctg gagcctataa tggaactggg acgaggtgcg tacggggtgg
      301 tggagaagat gcggcacgtg cccagcgggc agatcatggc agtgaagcgg atccgagcca
      361 cagtaaatag ccaggaacag aaacggctac tgatggattt ggatatttcc atgaggacgg
      421 tggactgtcc attcactgtc accttttatg gcgcactgtt tcgggagggt gatgtgtgga
      481 tctgcatgga gctcatggat acatcactag ataaattcta caaacaagtt attgataaag
      541 gccagacaat tccagaggac atcttaggga aaatagcagt ttctattgta aaagcattag
      601 aacatttaca tagtaagctg tctgtcattc acagagacgt caagccttct aatgtactca
      661 tcaatgctct cggtcaagtg aagatgtgcg a
//
