LOCUS       sequence-110720-         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Measurements of translational accuracy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   O'Connor M, Dahlberg AE
  TITLE     Mutations at U2555, a tRNA-protected base in 23S rRNA, affect 
            translational fidelity.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8.
  PUBMED    8415679
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-10-1"; volume: "90(19)"; pages: 
            "9214-8"
COMMENT     Sequence Label: pSG lacZ
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     145..162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     146..162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(219..241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    230..246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(254..260,261..276,277..282))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 aagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
       61 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      121 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattagg gccgctaacc
      181 ctacacaaag cttagcgtaa tcatggtgaa ttctgtttcc tgtgtgaaat tgttatccgc
      241 tcacaattgt cgacattata cgagccgatg attaattgtc aacacggccg acgcgctggg
      301 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
      361 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
      421 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
      481 tggaccgctg atcgtcacgg cgatttatgc cgcctcggc
//
