LOCUS       sequence-110723-         564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Detect translation initiation from GUG start codon (E. coli lacZ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 564)
  AUTHORS   O'Connor M, Thomas CL, Zimmermann RA, Dahlberg AE
  TITLE     Decoding fidelity at the ribosomal A and P sites: influence of 
            mutations in three different regions of the decoding domain in 16S 
            rRNA.
  JOURNAL   Nucleic Acids Res. 1997 Mar 15;25(6):1185-93.
  PUBMED    9092628
REFERENCE   2  (bases 1 to 564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1997-03-15"; volume: "25(6)"; pages: "1185-93"
COMMENT     Sequence Label: pSG 431
FEATURES             Location/Qualifiers
     source          1..564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     138..160
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     152..169
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     153..169
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(204..226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    215..231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(239..245,246..261,262..267))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccaggcaaag cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
       61 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
      121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttag
      181 cgtaatcacg gtgaattctg tttcctgtgt gaaattgtta tccgctcaca attgtcgaca
      241 ttatacgagc cgatgattaa ttgtcaacac ggccgacgcg ctgggctacg tcttgctggc
      301 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
      361 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
      421 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
      481 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
      541 cgccctatac cttgtctgcc tccc
//
