LOCUS       sequence-110727-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Gli2 truncation-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg caaggtcatc
      121 atgttcagga acttgctctc ctcggcgagg ctggtgagca tggagctcat gtccccgaca
      181 gccatgttgc tgatgcctgc ggggatggag ggcagggtca aggagtttcg gggggtggtg
      241 aggcgggagg agttctggga gaggctgtgg aggaggctgg ggctcagagc acccgagaac
      301 aaactcgagt gatcgccatc atccatgatg gcatcgaagt caatctgggg ggcctccagg
      361 agctgggagt ccacagtgct ggacacctgg ttgacccctg gtgagggcaa gggctggggc
      421 tggatgctgt cctgacaggc ctgtggctgt ggtggctggg accgcatgac ctggcagctc
      481 ccgaggttct ccaggcctcc atcctgttcg tacatgtgga tctggccgta gtagtagagc
      541 atgctgtggt ccggggcccc acctgcgtcc tgcggaggcg gctggggagg caccgacccc
      601 atgttgccca tcgctccctt gggcacagcc tctgctgttt cctgactgga cggcatggca
      661 gccatggcat ggcctgtggc cctggcgta
//
