LOCUS       sequence-110728-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Measurements of translational accuracy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   O'Connor M, Dahlberg AE
  TITLE     Mutations at U2555, a tRNA-protected base in 23S rRNA, affect 
            translational fidelity.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8.
  PUBMED    8415679
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-10-1"; volume: "90(19)"; pages: 
            "9214-8"
COMMENT     Sequence Label: pSG 853
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..161
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     153..170
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     154..170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(226..248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    237..253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(261..267,268..283,284..289))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
       61 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      121 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattagggc
      181 cgctaactta gacaaagctt agcgtaatca tggtgaattc tgtttcctgt gtgaaattgt
      241 tatccgctca caattgtcga cattatacga gccgatgatt aattgtcaac acggccgacg
      301 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
      361 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
      421 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
      481 cgatcattgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
      541 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
      601 gtgcatggag ccgggccacc tcgacctgaa tggaagccac tgcgatgagt gg
//
