LOCUS       sequence-110729-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Measurements of translational accuracy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   O'Connor M, Dahlberg AE
  TITLE     Mutations at U2555, a tRNA-protected base in 23S rRNA, affect 
            translational fidelity.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9214-8.
  PUBMED    8415679
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-10-1"; volume: "90(19)"; pages: 
            "9214-8"
COMMENT     Sequence Label: pSG 3/4
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     146..168
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     160..177
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     161..177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(245..267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    256..272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(280..286,287..302,303..308))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
       61 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      121 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      181 ttcccgggga tccgtcgtag tcaaagatac cctaagctta gcgtaatcat ggtgaattct
      241 gtttcctgtg tgaaattgtt atccgctcac aattgtcgac attatacgag ccgatgatta
      301 attgtcaaca cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
      361 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
      421 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
      481 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
      541 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
      601 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccact
      661 gcgatgagtg gcagggcgg
//
