LOCUS       sequence-110731-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Detect translation initiation from AUC start codon (E. coli lacZ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   O'Connor M, Thomas CL, Zimmermann RA, Dahlberg AE
  TITLE     Decoding fidelity at the ribosomal A and P sites: influence of 
            mutations in three different regions of the decoding domain in 16S 
            rRNA.
  JOURNAL   Nucleic Acids Res. 1997 Mar 15;25(6):1185-93.
  PUBMED    9092628
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1997-03-15"; volume: "25(6)"; pages: "1185-93"
COMMENT     Sequence Label: pSG 415
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..161
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     153..170
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     154..170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(205..227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    216..232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(240..246,247..262,263..268))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
       61 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      121 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctta
      181 gcgtaatgat ggtgaattct gtttcctgtg tgaaattgtt atccgctcac aattgtcgac
      241 attatacgag ccgatgatta attgtcaaca cggccgacgc gctgggctac gtcttgctgg
      301 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
      361 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
      421 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
      481 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
      541 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
      601 cgacctgaat ggaagccact gcgatgagtg gcagggcggg gcgtaatt
//
