LOCUS       sequence-110738-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible scrambled shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS,
            Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt 
            D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, 
            Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu 
            TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, 
            Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, 
            Brock MV, Seshagiri S
  TITLE     Comprehensive genomic analysis identifies SOX2 as a frequently 
            amplified gene in small-cell lung cancer.
  JOURNAL   Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 
            Sep 2.
  PUBMED    22941189
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2405"; 
            journalName: "Nat Genet"; date: "2012-10"; volume: "44"; issue: 
            "10"; pages: "1111-6"
COMMENT     Sequence Label: Tet-pLKO-puro-Scrambled
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    140..257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(357..451)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    357..375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    385..403
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(421..440)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg
       61 gctgccttgg aaaaggcgca accccaaccc cctcgagccg cggccaaagt ggatctctgc
      121 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
      181 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
      241 ccccttttct tttaaaattg tggatgaata ctgccatttg tctcgaggtc gagaattaaa
      301 aacctaaggt taagtcgccc tcgctcgagc gagggcgact taaccttagg ccggtgtctc
      361 tatcactgat agggaactta taagtctcta tcactgatag ggatttcacg tttatggtga
      421 tttcccagaa cacatagcga catgcaaata ttgcggccgc tcgaggctag tctcgtgatc
      481 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
      541 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
      601 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
      661 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
      721 ttatgttaaa ccaattccac aaacttgccc atttatctaa
//
