LOCUS       sequence-110741-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible scrambled shRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS,
            Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt 
            D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, 
            Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu 
            TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, 
            Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, 
            Brock MV, Seshagiri S
  TITLE     Comprehensive genomic analysis identifies SOX2 as a frequently 
            amplified gene in small-cell lung cancer.
  JOURNAL   Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 
            Sep 2.
  PUBMED    22941189
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2405"; 
            journalName: "Nat Genet"; date: "2012-10"; volume: "44"; issue: 
            "10"; pages: "1111-6"
COMMENT     Sequence Label: Tet-pLKO-puro-Scrambled
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             38..658
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(111..130)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(860..877)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aagaattgta atacgactca ctatagggcg aattgatatg tctagattag ataaaagtaa
       61 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
      121 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
      181 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
      241 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
      301 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
      361 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
      421 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
      481 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
      541 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
      601 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
      661 gtacagcggc tcccgggagt tctagggatc tgcccctctc cctccccccc ccctaacgtt
      721 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
      781 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
      841 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
      901 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cc
//
