LOCUS       sequence-110745-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible knockdown of SOX2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS,
            Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt 
            D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, 
            Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu 
            TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, 
            Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, 
            Brock MV, Seshagiri S
  TITLE     Comprehensive genomic analysis identifies SOX2 as a frequently 
            amplified gene in small-cell lung cancer.
  JOURNAL   Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 
            Sep 2.
  PUBMED    22941189
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2405"; 
            journalName: "Nat Genet"; date: "2012-10"; volume: "44"; issue: 
            "10"; pages: "1111-6"
COMMENT     Sequence Label: Tet-pLKO-puro-SOX2
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4..22
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             33..653
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(106..125)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ttgtaatacg actcactata gggcgaattg atatgtctag attagataaa agtaaagtga
       61 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
      121 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
      181 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
      241 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
      301 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
      361 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
      421 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
      481 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
      541 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
      601 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca
      661 gcggctcccg ggagttctag ggatctgccc ctctccctcc ccccccccta acgttactgg
      721 ccgaagccgc ttggaataa
//
