LOCUS       sequence-110747-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible knockdown of SOX2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS,
            Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt 
            D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, 
            Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu 
            TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, 
            Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, 
            Brock MV, Seshagiri S
  TITLE     Comprehensive genomic analysis identifies SOX2 as a frequently 
            amplified gene in small-cell lung cancer.
  JOURNAL   Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 
            Sep 2.
  PUBMED    22941189
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2405"; 
            journalName: "Nat Genet"; date: "2012-10"; volume: "44"; issue: 
            "10"; pages: "1111-6"
COMMENT     Sequence Label: Tet-pLKO-puro-SOX2
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    128..245
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(345..439)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    345..363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    373..391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(409..428)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(728..772)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttcccggaa ccacgcccag agcagccgcg tccctgcgca aacccagggc tgccttggaa
       61 aaggcgcaac cccaaccccc tcgagccgcg gccaaagtgg atctctgctg tccctgtaat
      121 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
      181 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
      241 taaaattgtg gatgaatact gccatttgtc tcgaggtcga gaattaaaaa ctgccgagaa
      301 tccatgtata tctcgagata tacatggatt ctcggcagcc ggtgtctcta tcactgatag
      361 ggaacttata agtctctatc actgataggg atttcacgtt tatggtgatt tcccagaaca
      421 catagcgaca tgcaaatatt gcggccgctc gaggctagtc tcgtgatcga taccgtcgag
      481 atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc
      541 cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat
      601 atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca
      661 agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc
      721 aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct
      781 ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt
      841 cccactccat ccaggtcgtg tgattccaaa tctgttccag a
//
