LOCUS       sequence-110756-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLIPQTV3.0 glutamine sensor with R75MW220A mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Gruenwald K, Holland JT, Stromberg V, Ahmad A, Watcharakichkorn D, 
            Okumoto S
  TITLE     Visualization of glutamine transporter activities in living cells 
            using genetically encoded glutamine sensors.
  JOURNAL   PLoS One. 2012;7(6):e38591. doi: 10.1371/journal.pone.0038591. Epub 
            2012 Jun 14.
  PUBMED    22723868
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(6):e38591. doi"; pages: " 
            10.1371/journal.pone.0038591. Epub 2012 Jun 14"
COMMENT     Sequence Label: pcDNA3.1(-) FLIPQTV3.0 2m
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             257..964
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagact
      121 cgagcggccg ccactgtgct ggatatctgc agaattccac cacactggac tagtggatcc
      181 actcgagatg gcaggctcga aattagttgt cgcgacggat accgccttcg ttccgttcga
      241 atttaaacag gccccaatgg tgagcaaggg cgaggagacc acaatgggcg taatcaagcc
      301 cgacatgaag atcaagctga agatggaggg caacgtgaat ggccacgcct tcgtgatcga
      361 gggcgagggc gagggcaagc cctacgacgg caccaacacc atcaacctgg aggtgaagga
      421 gggagccccc ctgcccttct cctacgacat tctgaccacc gcgttcgcct acggcaacag
      481 ggccttcacc aagtaccccg acgacatccc caactacttc aagcagtcct tccccgaggg
      541 ctactcttgg gagcgcacca tgaccttcga ggacaagggc atcgtgaagg tgaagtccga
      601 catctccatg gaggaggact ccttcatcta cgagatacac ctcaagggcg agaacttccc
      661 ccccaacggc cccgtgatgc agaagaagac caccggctgg gacgcctcca ccgagaggat
      721 gtacgtgcgc gacggcgtgc tgaagggcga cgttaagcac aagctgctgc tggagggcgg
      781 cggccaccac cgcgttgact tcaagaccat ctacagggcc aagaaggcgg tgaagctgcc
      841 cgactatcac tttgtggacc accgcatcga gatcctgaac cacgacaagg actacaacaa
      901 ggtgaccgtt tacgagagcg ccgtggcccg caactccacc gacggcatgg acgagctgta
      961 caaggtgagc gccaaatatg tgggctttga cgtcgatctg tgggctgcca tcgctaaaga
     1021 gctgaagctg
//
