LOCUS       sequence-110761-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLIPQTV3.0 glutamine sensor with R75MW220A mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Gruenwald K, Holland JT, Stromberg V, Ahmad A, Watcharakichkorn D, 
            Okumoto S
  TITLE     Visualization of glutamine transporter activities in living cells 
            using genetically encoded glutamine sensors.
  JOURNAL   PLoS One. 2012;7(6):e38591. doi: 10.1371/journal.pone.0038591. Epub 
            2012 Jun 14.
  PUBMED    22723868
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(6):e38591. doi"; pages: " 
            10.1371/journal.pone.0038591. Epub 2012 Jun 14"
COMMENT     Sequence Label: pcDNA3.1(-) FLIPQTV3.0 2m
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..264)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(55..76)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     404..423
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     663..684
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cttaagcttt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
       61 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta
      121 gctcaggtag tgattgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      181 gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct tgaagttggc
      241 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctgt tgtagttgta
      301 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      361 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      421 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      481 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt agcccagggt
      541 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatca gcttcagggt
      601 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      661 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cagcgccttt
      721 cggttcagta ccgaacgctt ttttgtagat ttcgttgtaa gttccgttct cgcgcagggt
      781 tttcaacgcg ccgttgactt tgtcacgcag ctcgtcgcta cctttcggga acgcaatacc
      841 gtattgctgc gcttccagag agtcacctac cgctttgaac tgaccg
//
