LOCUS       sequence-110764-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating p65AD hemidrivers from MiMIC lines with inserts into coding
            introns in Phase 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..118
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             286..339
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             370..390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gactcactat agggcgaatt ggagctcgct agcgatgtag gtcacggtct cgaagccgcg
       61 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
      121 gtccatcatg atggtcgact ctagaagtcg atccaacatg gcgacttgtc ccatccccgg
      181 catgtttaaa tatactaatt attcttgaac taattttaat caaccgattt atctctcttc
      241 cgcaggtcgg gaggttccgg tggaagcgga ggtagcggcg gatccgaggg ccgcggcagc
      301 ctgctgacct gcggcgatgt ggaggagaac cccgggccca tggataaagc ggaattaatt
      361 cccgagcctc caaaaaagaa gagaaaggtc gaattgggtt ccacgccgat ggagttccag
      421 tacctgcccg atacggatga ccgtcaccgt atcgaagaaa agcggaagcg aacctatgaa
      481 accttcaagt ccatcatgaa aaagtccccc ttctcgggcc ccacggaccc gcgccccccg
      541 ccccgtcgta ttgcggttcc ttcgcgcagc agtgccagcg tccccaaacc cgcaccgcag
      601 ccctacccgt tcacttcctc ccttagcacg attaactatg atgagttccc cacgatggtg
      661 ttccccagtg gacaaatttc ccaggcatcg gcactggctc cggccccacc gcaagtcctc
      721 ccccaggcgc ccgctccggc accggctccc gcaatggtga gtgctctggc ccaggccccc
      781 gctccagtcc ccgtgctggc gcctggaccc ccacaggcag ttgcccctcc tgctccgaaa
      841 ccaacgcagg cgggcgaagg aaccctgagc gaggccctct tgcagcttca gttcgatga
//
