LOCUS       sequence-110768-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLIPQTV3.0 glutamine sensor with R75MY86A mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Gruenwald K, Holland JT, Stromberg V, Ahmad A, Watcharakichkorn D, 
            Okumoto S
  TITLE     Visualization of glutamine transporter activities in living cells 
            using genetically encoded glutamine sensors.
  JOURNAL   PLoS One. 2012;7(6):e38591. doi: 10.1371/journal.pone.0038591. Epub 
            2012 Jun 14.
  PUBMED    22723868
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(6):e38591. doi"; pages: " 
            10.1371/journal.pone.0038591. Epub 2012 Jun 14"
COMMENT     Sequence Label: pcDNA3.1(-) FLIPQTV3.0 8m
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..257)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(48..69)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     397..416
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     656..677
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
       61 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg gtagctcagg
      121 tagtgattgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      181 tcggcgagct gcacgccgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg
      241 ccgttcttct gcttgtcggc ggtgatatag acgttgtggc tgttgtagtt gtactccagc
      301 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      361 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      421 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      481 tgcttcatgt ggtcggggta gcgggcgaag cactgcaggc cgtagcccag ggtggtcacg
      541 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tcagcttcag ggtcagcttg
      601 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      661 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcagcgcc tttcggttca
      721 gtaccgaacc attttttgta gatttcgttg taagttccgt tctcgcgcag ggttttcaac
      781 gcgccgttga ctttgtcacg cagctcgtcg ctacctttcg ggaacgcaat accgtattgc
      841 tgcgcttcca gagagtcacc taccgctttg aactgaccgt taccggcggt tttgatgaag
      901 tacagaatgt ttggcgtatc
//
