LOCUS       sequence-110774-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Plasmodium falciparum HGXPRT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Hazleton KZ, Ho MC, Cassera MB, Clinch K, Crump DR, Rosario I Jr, 
            Merino EF, Almo SC, Tyler PC, Schramm VL
  TITLE     Acyclic immucillin phosphonates: second-generation inhibitors of 
            Plasmodium falciparum hypoxanthine-guanine-xanthine 
            phosphoribosyltransferase.
  JOURNAL   Chem Biol. 2012 Jun 22;19(6):721-30. doi: 
            10.1016/j.chembiol.2012.04.012.
  PUBMED    22726686
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2012.04"; journalName: "Chem Biol"; date: 
            "2012-06-22- 22"; volume: "19"; issue: "6"; pages: "721-30"
COMMENT     Sequence Label: HGXPRT
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(798..822)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaagcaggct tctagaaaga acaggaggaa ttaaccatgg gcggttccca tcaccaccat
       61 caccacggcg gcctggtacc tcgcggctct catatgccga tcccgaataa cccgggtgct
      121 ggcgagaacg ctttcgatcc ggtgtttgtg aacgacgacg atggctacga tctggactcc
      181 tttatgattc cggcacatta caaaaagtac ctgactaaag tactggtacc gaacggtgtc
      241 attaaaaacc gtatcgaaaa gctggcttat gacattaaga aggtatacaa caacgaagaa
      301 tttcacatcc tgtgcctgct gaaaggttct cgtggttttt ttactgcgct gctgaaacac
      361 ctgagccgca tccacaacta cagcgcagtt gaaacttcca aaccactgtt tggcgaacat
      421 tacgtccgcg tgaagagcta ctgcaacgac cagtccactg gcacgctgga aattgtcagc
      481 gaagatctga gctgcctgaa aggcaagcat gttctgatcg ttgaggacat catcgacacc
      541 ggtaaaaccc tggtaaaatt ctgcgaatac ctgaaaaagt tcgaaatcaa aaccgtggct
      601 atcgcttgcc tgttcattaa acgtactccg ctgtggaacg gtttcaaggc ggatttcgtg
      661 ggctttagca tcccggacca tttcgtagtt ggttatagcc tggactacaa tgaaattttc
      721 cgtgacctgg accattgctg cctggttaac gacgaaggta agaaaaagta caaagctacc
      781 tctctgtaat aggatccacc cagctttctt gtacaaagtg gtgatgatcc ggctgctaac
      841 aaagcccgaa
//
