LOCUS       sequence-110781-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human Purine Nucleoside Phosphorylase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Lewandowicz A, Tyler PC, Evans GB, Furneaux RH, Schramm VL
  TITLE     Achieving the ultimate physiological goal in transition state 
            analogue inhibitors for purine nucleoside phosphorylase.
  JOURNAL   J Biol Chem. 2003 Aug 22;278(34):31465-8. Epub 2003 Jul 3.
  PUBMED    12842889
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2003-08-22"; volume: "278(34)"; pages: "31465-8. Epub
            2003 Jul 3"
COMMENT     Sequence Label: Human PNP his-tagged
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             50..82
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(86..94,95..109)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agaaggagat atacatatgc ggggttctca tcatcatcat catcatggta tggctagcat
       61 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccaaccct
      121 tatggagaac ggatacacct atgaagatta taagaacact gcagaatggc ttctgtctca
      181 tactaagcac cgacctcaag ttgcaataat ctgtggttct ggattaggag gtctgactga
      241 taaattaact caggcccaga tctttgacta cagtgaaatc cccaactttc ctcgaagtac
      301 agtgccaggt catgctggcc gactggtgtt tgggttcctg aatggcaggg cctgtgtgat
      361 gatgcagggc aggttccaca tgtatgaagg gtacccactc tggaaggtga cattcccagt
      421 gagggttttc caccttctgg gtgtggacac cctggtagtc accaatgcag caggagggct
      481 gaaccccaag tttgaggttg gagatatcat gctgatccgt gaccatatca acctacctgg
      541 tttcagtggt cagaaccctc tcagagggcc caatgatgaa aggtttggag atcgtttccc
      601 tgccatgtct gatgcctacg accggactat gaggcagagg gctctcagta cctggaaaca
      661 aatgggggag caacgtgagc tacaggaagg cacctatgtg atggtggcag gccccagctt
      721 tgagactgtg gcagaatgtc gtgtgctgca gaagctggga gcagacgctg ttggcatgag
      781 tacagtacca gaagttatcg ttgcacggca ctgtggactt cgagtctttg gcttctcact
      841 catcactaac aaggtcatca tggattatga aagcctggag aa
//
