LOCUS       sequence-110786-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLIPQTV3.0 glutamine sensor with D157N mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Gruenwald K, Holland JT, Stromberg V, Ahmad A, Watcharakichkorn D, 
            Okumoto S
  TITLE     Visualization of glutamine transporter activities in living cells 
            using genetically encoded glutamine sensors.
  JOURNAL   PLoS One. 2012;7(6):e38591. doi: 10.1371/journal.pone.0038591. Epub 
            2012 Jun 14.
  PUBMED    22723868
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(6):e38591. doi"; pages: " 
            10.1371/journal.pone.0038591. Epub 2012 Jun 14"
COMMENT     Sequence Label: pcDNA3.1(-) FLIPQTV3.0 100micro
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..256)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(47..68)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     396..415
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     655..676
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
       61 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt
      121 agtgattgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      181 cggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
      241 cgttcttctg cttgtcggcg gtgatataga cgttgtggct gttgtagttg tactccagct
      301 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      361 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      421 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      481 gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagcccagg gtggtcacga
      541 gggtgggcca gggcacgggc agcttgccgg tggtgcagat cagcttcagg gtcagcttgc
      601 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
      661 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcagcgcct ttcggttcag
      721 taccgaacca ttttttgtag atttcgttgt aagttccgtt ctcgcgcagg gttttcaacg
      781 cgccgttgac tttgtcacgc agctcgtcgc tacctttcgg gaacgcaata ccgtattgct
      841 gcgcttccag agagtcacct accgcttt
//
