LOCUS       sequence-110788-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-EFS-dCas9-GFP
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(83..94)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgtgatca gtttggcgtt cagcagctgc cgccaataat ttttcatttt cttgacaact
       61 tcttctgagg ggacgttatc actcttccct ctatttttat cggatcttgt caacacttta
      121 ttatcaatag aatcatcttt gagaaagctt tggggcacga tagcatccac gtcgtagtcg
      181 gacaaccggt tgatgtccag ttcctgatcc acgtacatgt ccctgccgtt ctgcaggtag
      241 tacaggtaga gcttctcatt ctgaagctgg gtgttttcaa ctgggtgttc cttaaggatt
      301 tgggacccca gttcttttat accctcttca atcctcttca tcctttccct actgttcttc
      361 tgtcccttct gggtagtttg gttctctcgg gccatctcga taacgatatt ctcgggctta
      421 tgccttccca ttactttgac gagttcatcc acgaccttaa cggtctgcag tattcccttt
      481 ttgatagctg ggctacctgc aagattagcg atgtgctcgt gaagactgtc cccctggcca
      541 gaaacttgtg ctttctggat gtcctcctta aaggtgagag agtcatcatg gatcaactgc
      601 atgaagttcc ggttggcaaa tccatcggac ttaagaaaat ccaggattgt ctttccactc
      661 tgcttgtctc ggatgccatt gatcagtttt cttgacagcc gcccccatcc tgtatatcgg
      721 cgtctcttga gctgtttcat gactttgtcg tcgaagagat gagcgtaagt tttcaagcgt
      781 tcttcaatca tctccctatc ttcaaacaac g
//
