LOCUS       sequence-110797-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating Gal4 drivers from MiMIC lines with inserts into coding 
            introns in Phase 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(2)-T2A-Gal4
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..119
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             287..340
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             341..781
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcgc tagcgatgta ggtcacggtc tcgaagccgc
       61 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
      121 ggtccatcat gatggtcgac tctagaagtc gatccaacat ggcgacttgt cccatccccg
      181 gcatgtttaa atatactaat tattcttgaa ctaattttaa tcaaccgatt tatctctctt
      241 ccgcaggtcg ggaggttccg gtggaagcgg aggtagcggc ggatccgagg gccgcggcag
      301 cctgctgacc tgcggcgatg tggaggagaa ccccgggccc atgaagctgc tgagcagcat
      361 cgagcaggcc tgcgatatct gccgcctgaa gaagctgaag tgcagcaagg agaagcccaa
      421 gtgcgccaag tgcctgaaga acaactggga gtgccgctac agccccaaga ccaagcgcag
      481 ccccctgacc cgcgcccacc tgaccgaggt ggagagccgc ctggagcgcc tggagcagct
      541 gttcctgctg atcttccccc gcgaggatct ggatatgatc ctgaagatgg atagcctgca
      601 ggatatcaag gccctgctga ccggcctgtt cgtgcaggat aacgtgaaca aggatgccgt
      661 gaccgatcgc ctggccagcg tggagaccga tatgcccctg accctgcgcc agcaccgcat
      721 cagcgccacc agcagcagcg aggagagcag caacaagggc cagcgccagc tgaccgtgag
      781 catcgatagc gccgcccacc acgataacag caccatcccc ctggatttca tgccccgcga
      841 tgccctgcac ggcttc
//
