LOCUS       sequence-110798-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRuby-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(245..280)
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     regulatory      322..331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actgcatgcc ctcatctagc gtctcaggga acatggcagc tcggaccctc tgagccctag
       61 tcattgcata ctgcccgtca atatcagcta cttgctcttg cgtgaaggac tgggaaaagc
      121 cttgctccca ttcataaagg acttgggagg tgtcaacatc ttcttcctca gggttgccct
      181 tgccactcag ggaaggagct gtggtggtgg caccagaatg gattccagaa tccaagtaag
      241 actgctgctg ccagtggctg acagcagctt ttctgtccgg ctccatggcc atgtccaact
      301 ccatcaggtc agcttgagta gccatggtgg ccgcggtacc gtcgactgca gaattcgaag
      361 cttgagctcg agatctgagt ccggaccctc cgcccaggcc ggcgaacttg gccacggcgt
      421 gctcccgctg caccacgaac atctcgttgt cggactcttc cagcctttcc agccggtggt
      481 ccacggcgtg gatgccgggc atcttgatgt tgcccacggt tttcttggat cggtaggtgg
      541 tcacgaagct gcagctcagg tgccctccgc cgtccacctt cagggccatg tgggtgtagc
      601 ctctcagtcc gccgtcagcg gggtacatca tctcggtgtt gggctcccag cccttggttt
      661 tcttctgcat cacggcgccg ttgctgggaa aattcacgcc cctgacctgg gcatggtaca
      721 ccaggcagcc atcttccagg ctggtgtcct gcatcacggt gatcacgccg ccgtcctcgt
      781 atctggtcac tctctcccag gtgaagccct cgggg
//
