LOCUS       sequence-110803-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating Gal80 lines from MiMIC lines with inserts into coding 
            introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..118
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             287..340
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gactcactat agggcgaatt ggagctcgct agcgatgtag gtcacggtct cgaagccgcg
       61 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
      121 gtccatcatg atggtcgact ctagaagtcg atccaacatg gcgacttgtc ccatccccgg
      181 catgtttaaa tatactaatt attcttgaac taattttaat caaccgattt atctctcttc
      241 cgcaggtggc ggaggttccg gtggaagcgg aggtagcggc ggatccgagg gccgcggcag
      301 cctgctgacc tgcggcgatg tggaggagaa ccccgggccc atggattaca acaaaaggag
      361 tagtgtgagt acggtgccga atgctgctcc cattcgcgtg ggcttcgtgg gattgaacgc
      421 ggctaagggt tgggccatta aaacgcatta tccagccata ctgcagctga gctcccagtt
      481 ccaaataaca gcgctttatt cccccaagat cgagacgtcc attgcgacaa ttcagaggct
      541 gaagttgtcc aacgccacag ctttcccaac actcgagagc ttcgcctcga gctccacgat
      601 cgatatgatt gtgatcgcaa tccaggtggc ttcccactac gaggtggtaa tgcccctgct
      661 cgagttttcg aagaacaatc ctaatctgaa gtatttgttt gtggaatggg cactggcctg
      721 ctcgctcgat caggcggagt cgatctataa ggccgctgca gagcggggcg tgcaaacaat
      781 aatcagtctg cagggacgaa agagcccgta cattttgcgg gcaaaagagc ttatctccca
      841 gggctacatc ggcgatataa atagcatcga gattgcaggt aacggaggtt ggtacggtta
//
