LOCUS       sequence-110806-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating p65AD hemidrivers from MiMIC lines with inserts into coding
            introns in Phase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..119
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             288..341
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             372..392
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcgc tagcgatgta ggtcacggtc tcgaagccgc
       61 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
      121 ggtccatcat gatggtcgac tctagaagtc gatccaacat ggcgacttgt cccatccccg
      181 gcatgtttaa atatactaat tattcttgaa ctaattttaa tcaaccgatt tatctctctt
      241 ccgcaggtgg cggaggttcc ggtggaagcg gaggtagcgg cggatccgag ggccgcggca
      301 gcctgctgac ctgcggcgat gtggaggaga accccgggcc catggataaa gcggaattaa
      361 ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg ttccacgccg atggagttcc
      421 agtacctgcc cgatacggat gaccgtcacc gtatcgaaga aaagcggaag cgaacctatg
      481 aaaccttcaa gtccatcatg aaaaagtccc ccttctcggg ccccacggac ccgcgccccc
      541 cgccccgtcg tattgcggtt ccttcgcgca gcagtgccag cgtccccaaa cccgcaccgc
      601 agccctaccc gttcacttcc tcccttagca cgattaacta tgatgagttc cccacgatgg
      661 tgttccccag tggacaaatt tcccaggcat cggcactggc tccggcccca ccgcaagtcc
      721 tcccccaggc gcccgctccg gcaccggctc ccgcaatggt gagtgctctg gcccaggccc
      781 ccgctccagt ccccgtgctg gcgcctggac ccccacaggc agttgcccct cctgctccga
      841 aaccaacgca ggcgggcgaa ggaaccctg
//
