LOCUS       sequence-110812-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating QF2 drivers from MiMIC lines with inserts into coding 
            introns in Phase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..118
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             287..340
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gactcactat agggcgaatt ggagctcgct agcgatgtag gtcacggtct cgaagccgcg
       61 gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg
      121 gtccatcatg atggtcgact ctagaagtcg atccaacatg gcgacttgtc ccatccccgg
      181 catgtttaaa tatactaatt attcttgaac taattttaat caaccgattt atctctcttc
      241 cgcaggtggc ggaggttccg gtggaagcgg aggtagcggc ggatccgagg gccgcggcag
      301 cctgctgacc tgcggcgatg tggaggagaa ccccgggccc atgccaccca agcgcaaaac
      361 gcttaacgct gcggctgagg ctaacgctca tgccgacgga cacgccgacg gaaacgccga
      421 cggacacgtg gccaatacgg ccgcgtcctc gaataatgcg aggttcgctg atctcactaa
      481 catcgatact ccgggtctgg gacccacaac tacgaccctg ctcgtggaac cagcacgctc
      541 aaagcgtcaa cgagtgtccc gcgcatgcga ccagtgccgt gcagcccgag agaaatgcga
      601 cggaatacag cctgcgtgtt tcccgtgcgt ttcccaggga aggtcctgca cttatcaggc
      661 ttcgccgaaa aagaggggag ttcaaaccgg ttatattcgt acgctggagc tcgccctcgc
      721 ctggatgttt gaaaatgtcg cgcgttccga agatgccttg cataacctcc tcgtccgtga
      781 cgccggacaa ggatcagctc tgctcgttgg taaagattcg ccggctgccg agcgactcca
      841 tgcccgttgg gctactagcc gtgtcaataa gagcattacc cgcctcctcc gtcagttgga
      901 gctccctcct accgccacgg ctacggcctc gataatgccg cacgtgatgg agcagcctct
      961 cagtaccagc attaaccccg tcaa
//
