LOCUS       sequence-110817-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human Phenylethanolamine N-methyltransferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Stratton CF, Poulin MB, Du Q, Schramm VL
  TITLE     Kinetic Isotope Effects and Transition State Structure for Human 
            Phenylethanolamine N-Methyltransferase.
  JOURNAL   ACS Chem Biol. 2017 Feb 17;12(2):342-346. doi: 
            10.1021/acschembio.6b00922. Epub 2016 Dec 28.
  PUBMED    27997103
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.6b00922"; journalName: "ACS Chem Biol"; date: 
            "2017-02-17- 17"; volume: "12"; issue: "2"; pages: "342-346"
COMMENT     Sequence Label: PNMT
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta acttttagga ggtaaaacat atgggttctt cgcatcacca ccatcatcat
       61 tctagcggtc tggttccacg tggcagcatg agcggtgctg atcgtagccc gaacgccggt
      121 gcggcaccgg acagcgcacc gggtcaggcc gctgtggcga gcgcgtatca acgcttcgag
      181 cctcgcgcat atttgcgcaa taactacgca ccgcctcgtg gtgatctgtg taacccgaac
      241 ggcgtcggtc cgtggaagct gcgctgcctg gcgcagacgt tcgcgaccgg cgaggtgagc
      301 ggtcgtacct tgattgatat cggtagcggc ccaaccgtct atcaactgct gagcgcgtgc
      361 agccactttg aagatattac gatgaccgac ttcctggaag tgaatcgtca agaactgggt
      421 cgttggctgc aagaggaacc gggtgccttc aattggtcca tgtactccca gcatgcgtgc
      481 ctgattgagg gtaagggcga gtgttggcag gacaaggaac gccagctgcg tgcgcgcgtg
      541 aaacgtgtcc tgccgatcga tgtccaccaa ccgcagccgc tgggtgcggg tagcccggct
      601 ccgctgccgg cagacgccct ggttagcgcg ttttgtcttg aggccgtcag cccggacttg
      661 gcgagctttc agcgtgctct ggatcacatc acgactctgc tgcgtccggg tggccacctg
      721 ttgctgattg gtgccctgga agagtcctgg tacttggcag gcgaggcacg tctgacggta
      781 gttccggtga gcgaagaaga ggttcgtgaa gcgctggtgc gttcaggcta caaagttcgc
      841 gatctgagaa cctacatcat gccggcacac ttacagaccg gtgttgacga cgtta
//
