LOCUS       sequence-110824-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation:N/A, 
            Emission:N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-TagRFP-pBad
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..62)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(75..107)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(111..128)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             148..170
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(197..481)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(255..274)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ttctccttaa tcagctcttc gcccttagac accatgctcg agccctggaa gtacaggttc
       61 tcgtacagat cccgacccat ttgctgtcca ccagtcatgc tagccatacc atgatgatga
      121 tgatgatgag aaccccgcat atgtatatct ccttcttaaa gttaaacaaa attatttcta
      181 gcccaaaaaa acgggtatgg agaaacagta gagagttgcg ataaaaagcg tcaggtagta
      241 tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa
      301 tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt tgtcatggct
      361 ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttt ggttagcgag
      421 aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca attggtttct
      481 tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc cctgctgccg
      541 ggatactcgt ttaatgccca tctggtggcg ggtttaacgc cgattgaggc caacggttat
      601 ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt
      661 cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tttgccgacc gggtgatatt
      721 ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc tcgcgaatgg
      781 tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct taactggccg
      841 tcaatatttg ccaatacggg gttctttcgc ccggatgaag cgcac
//
