LOCUS       sequence-110847-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: TGFbetaR1 (T204D)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg caacattttg
      121 atgccttcct gttgactgag ttgcgataat gttttcttaa tccgcaatgc tgtaagccta
      181 gctgctccat tggcatacca acattctctc ataattttag ccattactct caaggcttca
      241 cagctctgcc atctgtttgg gatatttggc cttaacttct gttcacaaac aacttttctc
      301 atttcttcaa ctgatgggtc agaaggtaca agatcataat aaggcagttg gtaatcttca
      361 tgaattccac caatggaaca tcgtcgagca atttcccaga atactaagcc cattgcatag
      421 atgtcagcac gtttgaagga ttcaaaatgt ttcatattta tggaatcatc gagaacttca
      481 ggggccatgt acctttttgt tcccactctg tggtttggag caatatcaat ggtatctgtg
      541 gctgaatcat gtcttactgc cagtcctaag tctgcaatac agcaagttcc attcttcttt
      601 accaagatat tctttgattt caaatctcta tgagcaatgg ctggctttcc ttgggtacca
      661 acaatctcca tgtgaagatg ggcaagaccg ctcgccgtgg acagagcaag ttttatcatt
      721 ccttccacag taactgtgta tctg
//
