LOCUS       sequence-110865-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NQO1 Flag tagged at C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Tsvetkov P, Adamovich Y, Elliott E, Shaul Y
  TITLE     E3 ligase STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 
            (NQO1) accumulation in aged brain, a process impaired in certain 
            Alzheimer disease patients.
  JOURNAL   J Biol Chem. 2011 Mar 18;286(11):8839-45. doi: 
            10.1074/jbc.M110.193276. Epub 2011 Jan 10.
  PUBMED    21220432
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.193276"; journalName: "J Biol Chem"; date: 
            "2011-03-18- 18"; volume: "286"; issue: "11"; pages: "8839-45"
COMMENT     Sequence Label: pCDNA3 NQO1-Flag
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgccacca tggtcggcag aagagcactg atcgtactgg ctcactcaga
      121 gaggacctcc ttcaactatg ccatgaagga ggctgctgca gcggctttga agaagaaagg
      181 atgggaggtg gtggagtcgg acctctatgc catgaacttc aatcccatca tttccagaaa
      241 ggacatcaca ggtaaactga aggaccctgc gaactttcag tatcctgccg agtctgttct
      301 ggcttataaa gaaggccatc tgagcccaga tattgtggct gaacaaaaga agctggaagc
      361 cgcagacctt gtgatattcc agttccccct gcagtggttt ggagtccctg ccattctgaa
      421 aggctggttt gagcgagtgt tcataggaga gtttgcttac acttacgctg ccatgtatga
      481 caaaggaccc ttccggagta agaaggcagt gctttccatc accactggtg gcagtggctc
      541 catgtactct ctgcaaggga tccacgggga catgaatgtc attctctggc caattcagag
      601 tggcattctg catttctgtg gcttccaagt cttagaacct caactgacat atagcattgg
      661 gcacactcca gcagacgccc gaattcaaat cctggaagga tggaagaaac gcctggagaa
      721 tatttgggat gagacaccac tgtattttgc tccaagcagc ctctttgacc taaacttcca
      781 ggcaggattc ttaatgaaaa
//
