LOCUS       sequence-110869-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of E. coli DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Wang Z, Singh P, Czekster CM, Kohen A, Schramm VL
  TITLE     Protein mass-modulated effects in the catalytic mechanism of 
            dihydrofolate reductase: beyond promoting vibrations.
  JOURNAL   J Am Chem Soc. 2014 Jun 11;136(23):8333-41. doi: 10.1021/ja501936d. 
            Epub 2014 May 27.
  PUBMED    24820793
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja501936d";
            journalName: "J Am Chem Soc"; date: "2014-06-11- 11"; volume: "136";
            issue: "23"; pages: "8333-41"
COMMENT     Sequence Label: DHFR
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      515..562
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(787..806)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(812..831)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 acttttagga ggtaaaacat atgatgatta gcttgattgc agcactggcc gtcgaccgcg
       61 tgattggcat ggagaacgca atgccgtgga atctgcctgc ggacctggcg tggttcaaac
      121 gtaatactct ggacaagccg gtgattatgg gtcgtcatac ctgggagtcc attggtcgtc
      181 cactgccggg tcgcaaaaac atcattctga gcagccaacc gggtacggac gaccgcgtca
      241 cctgggtcaa gtctgtggac gaagcgatcg ccgcttgcgg cgatgtgccg gaaatcatgg
      301 ttatcggtgg cggtcgtgtt tatgagcagt ttctgccgaa ggctcaaaaa ctgtacttga
      361 cgcacatcga cgcggaggtt gaaggcgata cccacttccc ggattacgag ccggacgatt
      421 gggaatcggt ttttagcgaa tttcacgatg cggatgcgca gaacagccat agctattgtt
      481 tcgagatcct ggagcgtcgt tgataagctt ccccctagca taaccccttg gggcctctaa
      541 acgggtcttg aggggttttt tgcccctgag acgcgtcaat cgagttcgta cctaagggcg
      601 acacccccta attagcccgg ggcgaaaggc ccagtctttc gactgagcct ttcgttttta
      661 tttgatgcct ggcagttccc tactctcgca tggggagtcc ccacactacc atcggcgcta
      721 cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca
      781 ggcaaacaag gggtgttatg agccatattc aggtataaat gggctcgcga taatgttcag
      841 aattggttaa ttggttgtaa cactgacccc tatttgttta tttt
//
