LOCUS       sequence-110874-         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid. When used to produce 
            lentivirus, express physiological levels of insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: p53 (dominant negative R175H mutant)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacctctctc cccaggggct agcatcgatg gatcaacaag tttgtacaaa aaagttggct
       61 gcagcggcgg cggcaccatg gaggagccgc agtcagatcc tagcgtcgag ccccctctga
      121 gtcaggaaac attttcagac ctatggaaac tacttcctga aaacaacgtt ctgtccccct
      181 tgccgtccca agcaatggat gatttgatgc tgtccccgga cgatattgaa caatggttca
      241 ctgaagaccc aggtccagat gaagctccca gaatgccaga ggctgctccc cgcgtggccc
      301 ctgcaccagc agctcctaca ccggcggccc ctgcaccagc cccctcctgg cccctgtcat
      361 cttctgtccc ttcccagaaa acctaccagg gcagctacgg tttccgtctg ggcttcttgc
      421 attctgggac agccaagtct gtgacttgca cgtactcccc tgccctcaac aagatgtttt
      481 gccaactggc caagacctgc cctgtgcagc tgtgggttga ttccacaccc ccgcccggca
      541 cccgcgtccg cgccatggcc atctacaagc agtcacagca catgacggag gttgtgaggc
      601 actgccccca ccatgagcgc tgctcagata gcgatggtct ggcccctcct cagcatctta
      661 tccgagt
//
