LOCUS       sequence-110875-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid. When used to produce 
            lentivirus, express physiological levels of insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: p53 (dominant negative R175H mutant)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..85)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga gggttgattg tcgacttaac gcggtcatta ctacgtagaa tcgagaccga
       61 ggagagggtt agggataggc ttaccaccgg taaccacttt gtacaagaaa gttggcaagt
      121 ctgagtcagg cccttctgtc ttgaacatga gttttttatg gcgggaggta gactgaccct
      181 ttttggactt caggtggctg gagtgagccc tgctcccccc cggctccttc ccagcctggg
      241 catccttgag ttccaaggcc tcattcagct ctcggaacat ctcgaagcgc tcacgcccac
      301 ggatctgaag ggtgaaatat tctccatcca gtggtttctt ctttggctgg ggagaggagc
      361 tggtgttgtt gggcagtgct cgcttagtgc tccctggggg cagctcgtgg tgaggctccc
      421 ctttcttgcg gagattctct tcctctgtgc gccggtctct cccaggacag gcacaaacac
      481 gcacctcaaa gctgttccgt cccagtagat taccactgga gtcttccagt gtgatgatgg
      541 tgaggatggg cctccggttc atgccgccca tgcaggaact gttacacatg tagttgtagt
//
