LOCUS       sequence-110876-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: HRas (G12V, E37G)-pcw107
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(85..117,118..120,121..654)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIGDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(656..680)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaccgacct ctctccccag gggcctagca tcgaatggat caacaagttt gtacaaaaaa
       61 gttggcttcc gcggcggcgg caccatgacg gaatataagc tggtggtggt gggcgccgtc
      121 ggtgtgggca agagtgcgct gaccatccag ctgatccaga accattttgt ggacgaatac
      181 gaccccacta taggggattc ctaccggaag caggtggtca ttgatgggga gacgtgcctg
      241 ttggacatcc tggataccgc cggccaggag gagtacagcg ccatgcggga ccagtacatg
      301 cgcaccgggg agggcttcct gtgtgtgttt gccatcaaca acaccaagtc ttttgaggac
      361 atccaccagt acagggagca gatcaaacgg gtgaaggact cggatgacgt gcccatggtg
      421 ctggtgggga acaagtgtga cctggctgca cgcactgtgg aatctcggca ggctcaggac
      481 ctcgcccgaa gctacggcat cccctacatc gagacctcgg ccaagacccg gcagggagtg
      541 gaggatgcct tctacacgtt ggtgcgtgag atccggcagc acaagctgcg gaagctgaac
      601 cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc ctgattgcca
      661 actttcttgt acaaagtggt taccggttag taatgaacgc gttaagtcga caatcaacct
      721 ctggattac
//
