LOCUS       sequence-110877-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: HRas (G12V, E37G)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(83..115,116..118,119..649)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIGDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(651..675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             683..724
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcaccgacct ctctccccag gggctagcat cgatggatca acaagtttgt acaaaaaagt
       61 tggcttccgc ggcggcggca ccatgacgga atataagctg gtggtggtgg gcgccgtcgg
      121 tgtgggcaag agtgcgctga ccatccagct gatccagaac cattttgtgg acgaatacga
      181 ccccactata ggggattcct accggaagca ggtggtcatt gatggggaga cgtgcctgtt
      241 ggacatcctg gataccgccg gccaggagga gtacagcgcc atgcgggacc agtacatgcg
      301 caccggggag ggcttcctgt gtgtgtttgc catcaacaac accaagtctt ttgaggacat
      361 ccaccagtac agggagcaga tcaaacgggt gaaggactcg gatgacgtgc ccatggtgct
      421 ggtggggaac aagtgtgacc tggctgcacg cactgtggaa tctcggcagg ctcaggacct
      481 cgcccgaagc tacggcatcc cctacatcga gacctcggcc aagacccggc agggagtgga
      541 ggatgccttc tacacgttgg tgcgtgagat ccggcagcac aagctgcgga agctgaaccc
      601 tcctgatgag agtggccccg gctgcatgag ctgcaagtgt gtgctctcct tgccaacttt
      661 cttgtacaaa gtggttaccg gtggtaagcc tatccctaac cctctcctcg gtctcgattc
      721 tacgtagtaa tgaccgcgtt aagtcgacaa tcaacctctg gattac
//
