LOCUS       sequence-110880-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Rgl2-CAAX-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..84)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cggtcattac tacgtagaat cgagaccgag
       61 gagagggtta gggataggct taccaccggt aaccactttg tacaagaaag ttggcaacat
      121 aattacacac tttgtctttg acttcttttt cttcttttta ccatctttgc ttagacgcgt
      181 gaacagtgcc cgtgcaatct tcctccccgt ggccttgatc ctgggaaagg agccccctcc
      241 cccctcgctc ggaggagttc ctgaggcaga ggggccgctg tgggaacccg gggtggcagc
      301 agagggtctt ctgcgctgcc gcaggaggaa gtcatgagat gcaccatcca tggcatagaa
      361 gacgttagct gagtgtggga tggtcagctc tcgatcccca ggtagcagct gcaccagctc
      421 gaactctgaa gccacagcag aatcacgatt gtttttctta aggacacgac taataacact
      481 tggagctttg tcctggcttg tcaccaggat gctcttgtag acgctgccat cctcccccag
      541 ctccatctgg actcggatga ttcggcaatc agaggaccct ggcccagata ccccgccccc
      601 acatccagca ctcctagagg tcccttctcc tgtgtttcca ctcaacggag acccacagga
      661 ggctgagcga cggtga
//
