LOCUS       sequence-110894-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Ricin A Chain (RTA) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Ho MC, Sturm MB, Almo SC, Schramm VL
  TITLE     Transition state analogues in structures of ricin and saporin 
            ribosome-inactivating proteins.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20276-81. doi: 
            10.1073/pnas.0911606106. Epub 2009 Nov 17.
  PUBMED    19920175
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0911606106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-12-1- 1"; volume: "106"; issue: "48"; pages: "20276-81"
COMMENT     Sequence Label: RTA
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatcct attagaattg gctagacggt ggcggggcgc agcggtatac
      121 catcagtgca atgattggga tcaggatgct aacgtcgtac acgctaaatt tgctgccatt
      181 gcggcgttgc agttgaatag ggctagcgaa ggcgccctga ttgctctctt ggatcgcggt
      241 gctcagacgg ccccaggaat tttccagggt aataacagaa ggatctgggg cgctacggcg
      301 gttgtaacga atacgggtac gcatttcacc ttcaatatat tggaaacgtg ctgcttcaga
      361 gatcatttga atacagatga tgaagctacg cgccagcgtt ggcagctggg taccgcccgt
      421 ggagtagtag tacagcgcgc tgatggcttc ttccagtggg ccgttgccca gttcaatgtt
      481 ttcacgcaga ttaccagcca gctgctccag acgatcatag ttaccaccga aggcgaaggt
      541 ataacggttt tggacgtcgg taaacagatg ggtgattgct tcggcatcct cctggttatc
      601 tgggtgaaag aagtaggcgc tattaccggc gcgatagccg acaacatacg cattggtgac
      661 gtccagcgcc agggttacag acagctcggc atggttgctc agttctacca gaataaa
//
