LOCUS       sequence-110895-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  (control) when used to produce lentivirus, express physiological 
            levels of insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: RalA (G23V)( full )-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(703..727)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             735..776
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcaccgaacc tctctcccca ggggctagca tcgatggatc aaccagtttg tacaaaaaag
       61 ttggcttttg cggcggcggc accatggctg caaataagcc caagggtcaa aattctttgg
      121 ccttacacaa agtcatcatg gtgggcagtg ttggtgtggg caagtcagct ctgactctac
      181 agttcatgta cgatgagttt gtggaggact atgagcctac caaagcagac agctaccgga
      241 agaaggtagt gctggatggg gaggaagtac agattgatat cttagataca gctgggcagg
      301 aggactatgc tgcaattaga gacaactact tccgaagcgg ggagggcttc ctctgtgtct
      361 tctctattac agaaatggaa tcctttgcag ctacagctga cttcagggag cagattttaa
      421 gagtaaaaga agacgagaat gttccatttc tactggttgg taacaaatca gatttagaag
      481 ataaaaggca ggtttctgta gaagaggcaa aaaacagagc tgaccagtgg aatgttaact
      541 acgtggaaac gtctgctaaa acacgagcta atgttgacaa ggtatttttt gatttaatga
      601 gagaaattcg agcaagaaag atggaagaca gcaaagaaaa gaatggaaaa aagaagagga
      661 aaagtttagc caagagaatc agagaaagat gctgcatttt attgccaact ttcttgtaca
      721 aagtggttac cggtggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
      781 aatgaccgcg ttaagtcg
//
