LOCUS       sequence-110901-       12227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter 
            vector to select iChon cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N
  TITLE     Direct induction of chondrogenic cells from human dermal fibroblast 
            culture by defined factors.
  JOURNAL   PLoS One. 2013 Oct 16;8(10):e77365. doi: 
            10.1371/journal.pone.0077365. eCollection 2013.
  PUBMED    24146984
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-16"; volume: "8(10):e77365. doi"; pages: " 
            10.1371/journal.pone.0077365. eCollection 2013"
COMMENT     Sequence Label: pLe6-Delta -hLP-mcs/(EGFP-IresPuro-hInt)
FEATURES             Location/Qualifiers
     source          1..12227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1951..1971
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3180..3204
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3242..3251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3248..3250,3251..3253,3254..3967)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3248..3250,3251..3253,3254..3964)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3293..3314)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3554..3573)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3901..3922
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3994..4568,4569..4571,4572..4580)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4161..4178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4388..4407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4581..5183
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5080..5100
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7938..7962)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8015..8056
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8080..8668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8133..8153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8551..8562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8748..8981
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9053..9174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9090..9109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9144..9163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9214..9349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9369..9388)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9370..9388)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9398..9415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9398..9414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9407..9429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9556..10011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9643..9662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9853..9874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10037..10141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10142..10210,10211..11002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10360..10379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11173..11761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11662..11681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11915..11932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12049..12070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12085..12090,12091..12108,12109..12115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12123..12139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12128..12150
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12147..12163
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12147..12163
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12182..12202
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12184..12202
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatgtc gacgttaacg ctagtgatat caactttgta tagaaaagtt ggctccgaat
     1981 tcaatcgctg ttttctggac tgaggtgact gtatgaggat gggcagcgcc gtttcgggct
     2041 tggggggggt atccggttgg gagctccgga cgcgatccct ggacgccgcc gccgaggttc
     2101 catggggccc aagaggtgca cgcagggtgg gggacacaag gcaagctttg tcggggaggg
     2161 ggggagaggg tgtgccaggc tgggggcggg gccggccgga ggaggaaggg ggggcggtgg
     2221 attctcaaag gcgccttgtt cgctcgtgcc ctctccgcgc cagcgggcgg cggcgcctcg
     2281 gctcgctgcg gccttccctc cgggcgcgct gcgggctccg ggcagacccg gcgccgtgcc
     2341 cgctcgtggg ggcgcactgg gcgccgagcg ctgcgtgctt ctcaggcgtt gcccgcccgg
     2401 aggcggccca cgtccccgag tgaccccatt tccctggacc cttccaacca gagtcgactg
     2461 ctcgctcatc ttaccactgc tccccctcta cctcggctct gggactccct aaccgcgtcc
     2521 cccatttgca tctccttgga cctgactatc ctgtgtttgt cggtgggtcc cagtctctgg
     2581 cctcttgctc tccgtagaac accttccccc acatcccccg ccccagttcg tcgctatcta
     2641 tagccttgtc tataaatacc cccgccccgg cccggctctg taattacacg gggcggggtg
     2701 agggaagtaa ttatggagac ctgattatgg gtggggtggg gactgcgacc cccaggcccg
     2761 cctctccccc tcccactgtg ccctaaatcc cgcccaggcc tctgctgaaa gggggctctg
     2821 ggcccccaag agggagggaa tgggagggag tgtgtgtgac tggacgtttg ggtcctagaa
     2881 aaggaagggg ctagggaaga tattggggtt cccgaaaaga gaatcttagg gtacaggccg
     2941 ttgagaccta caaggggcag gagagagcga gcgatagagg gagggttccc gcctccctcc
     3001 ccaggtggag actgagggtg gggtttccct tcggtggctg tgggcgggcg gctggaggcg
     3061 ggggccgggc cgggggcggg ggcgaggtgg gggctctggg cgccagggtg gccggggaca
     3121 cacagaagcg gcagccaccg aggagggagc agtgccggga gctcgagtgt caattcgacc
     3181 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccaccggt
     3241 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3361 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3421 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3481 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3661 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3721 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3781 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3841 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3901 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3961 caagtaaagc ggccgcaagc tgagcaggat ccgcccctct ccctcccccc cccctaacgt
     4021 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4081 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4141 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4201 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4261 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4321 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4381 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4441 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4501 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4561 atgataatat ggccacaacc atggcaaccg agtacaagcc cacggtgcgc ctcgccaccc
     4621 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     4681 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     4741 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     4801 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     4861 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     4921 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     4981 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     5041 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     5101 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     5161 tgacccgcaa gcccggtgcc tgagcggccg caactagtcc tcgaggtaag aggagtcctg
     5221 atgcctgggt cctcggagtc aggctggagc tctgagtgtc tctgggaagg ggccagctga
     5281 tgcctggggc agcagcttct gagtctaaca aggagatttg gggcttcaag gctacaggct
     5341 ggagggcagg acacctgtgt tccttggcat cagggtagga atctctttgt ccttgaagtg
     5401 actgggggag ggcagatagg gctgaaaggt ggaggaccac tttagagacc ctgaatgggg
     5461 atggatgctt gtcttgagtc cttgggaaga gctgaagatg gccagggcca gcccggtcca
     5521 gtcattgctg gtgtggggat ggtagacgcc aatgttgatg ggtgaggcgt gtgtgtctta
     5581 tggcctttct gtcgatatct ctgggataag gggtgagcac ctgtgattca gagcagagcc
     5641 cagtaagccc agaaagaaag gaagccttga ctacctgtct cattggcctc ctggacaggg
     5701 tccccctccc cctccctgtg ccctcgatgc tgccacgcag ctggctctgg ggagcactgg
     5761 ggctggctgc caacaggacg gccctccgga caggccagga tcaggtgtct gaggccagag
     5821 ccaactcttt gcattcctgc ctagcccctc ctccctctgg ccagccaggc tcccctggga
     5881 ccctggtgcc cacaaccctg atcttttcct tctttttccc aggacccaga attcctggct
     5941 tctaggaaag gggatttgat gtcaaggagg gggctgtgag caccaggtcc tcagcaggct
     6001 ggaaaaagcc catttctgga gacactgagg gctgatttat atttaacttg actattcaat
     6061 tcttctctgc tttagagaga aaatggtcag aatggcagca caaaagattt agattagatt
     6121 acagaaggaa gaacttccaa gatgtgagga cagtgaagcc cagggccagg aaattgaggc
     6181 gtatggtgcc tgtagactct aggagtcttt cttaggggga agagggatcc tctggcatgg
     6241 aggcaggggg atggctaaga tgactgtttc ttatttcagg ggctgagtgg gtagagatgg
     6301 gactttaggg aaatgagtta ctgtagaatt cctaaggtta gagaataagg catttggggg
     6361 gccagaggta gagtggtgga gggtaatggg gctaccagtg gagagcctgt ggggcagatg
     6421 aggctggata caggggggca agtatgtggc ctggtgtggg aagatggtgg gcggacctgt
     6481 gggtaggttg tccatatgat cgaattgtac tcccttggga tttggtggag ggtggggacc
     6541 tccgaggtct ccttggcctg gggtctctgt gagtctctgt gtctctgtct cactcaccct
     6601 tcgtctctca aagcggccct tgtgtgttgg tgtttgcgga gctgccacac gcgcaggggc
     6661 caggcttagg tgggggttgg gggggaaggc cgtggtttcc cggctcctgg actcaaaggg
     6721 ccttttctct gcctgcccgc cccacctcac ccaccccacc cagctccact ctcctgattg
     6781 ctctggcctc tgccctgccc cacacttcta tgaaagtttt gctggaactc tctttgggat
     6841 ttttccaatc tggagctgat ggtgtgaaga gtatgtgtat aggggagcaa gtgagggcaa
     6901 ggtgatcttt tgttttccag ggagtttgag ccctaggaag ctgagttcta gttagacagg
     6961 gctggtgggc tggatcttga atctttgggt cccaggtggt ggggttggtg ggggtggtga
     7021 gacaggattg aggttccagg tgtggggagc ttgcctgggt tctgggcaag cactctgatg
     7081 ggatccacag atgtgtggct cagggccctg gacctgttcc ggctcctcgt ggcagcttca
     7141 gcccctgccc cccattcctc cacccctccc caactcatct cctaaaccag gaggcacaga
     7201 ctcctgggag agctttcttg tatctttttc ctcagcatgc tcagggttgt tcccacccca
     7261 tcccccaaac ctgggatctg tagctgcttg aagcccctct catagagctg cttctctgat
     7321 ctcttcctcg tggcccccct gcacctcctg tgtccaggat gatcgattgc ccagatgttg
     7381 tttgtgctgg agacgggaca gatgccagag acccagagag tgagagatat ggaaagagag
     7441 tgagagcgag ggagagaaaa agatggacag aatgtgagag aaatggagga agacagaaga
     7501 gacagagaca gaaagaccaa cataaagaga cagagagagt aagagagtga gagagacagg
     7561 agacagatga agagttgaga gaagtattca gagagtacag agagagacag attcataagc
     7621 taaaagacaa agagagagcg taagagaggc agatgctagg agagacaggg gcaggagtgt
     7681 catggggttg aatgccagct gatgctgccc tgcctccagg tacctttgca tttcccatcc
     7741 attcaggtac tgttcagaag caagtgaggg tgaggtgacc tttcccttgc agggagtttg
     7801 agccctggaa agctgagttc tagatcccaa atgtccccta tttatgccct cctgagggca
     7861 tgtcccttct cctgataatc atggactctc ccagagatct ggaagcttgg gaattcggca
     7921 tatgtcgaga tatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     7981 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     8041 cggtctcgat tctacgcgta ccggttagta atgatcgaca atcaacctct ggattacaaa
     8101 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8161 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8221 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8281 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8341 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8401 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8461 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     8521 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8581 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8641 cggatctccc tttgggccgc ctccccgcct ggcgatggta cctttaagac caatgactta
     8701 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8761 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8821 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8881 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8941 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     9001 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     9061 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9121 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9181 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9241 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9301 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     9361 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     9421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     9481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     9541 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     9601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     9661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     9721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     9781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     9841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     9901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
    10021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    10201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    10261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    10321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    10381 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    10441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    10501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    10561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    10621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    10681 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    11041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    11101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    11161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    11221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    11281 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    11341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    11401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    11461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    11521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    11581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    11641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    11701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    11821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    11881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    11941 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    12001 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    12061 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    12121 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    12181 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt
//
