LOCUS       sequence-110911-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses crtEBI genes for neurosporene production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: pBad_crtEBI_19
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             117..125
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctctgattag cgttctggct caactggagg ctcaggatcg tcagatcagc cgccagcgtc
       61 tgggtaaccg tgcgaacccg atctaactcg aagcttaaac ccaacaaatt agactataag
      121 gaggttaata atgccatcta tttctccagc ctctgacgct gatcgtgctc tggttatcgg
      181 ctctggcctg ggcggcctgg ccgctgctat gcgtctgggt gcaaaaggtt ggcgtgttac
      241 cgtaatcgat aaactggacg ttccgggtgg ccgtggctcc agcatcacgc aggagggcca
      301 tcgttttgac ctgggcccga ccattgttac tgtacctcaa agcctgcgtg acctgtggaa
      361 aacttgcggt cgcgacttcg acgcagatgt ggagctgaaa ccgattgacc cattctacga
      421 agttcgttgg ccagatggct ctcactttac cgttcgtcag tctactgaag ctatgaaagc
      481 tgaagtagca cgcctgtctc caggcgatgt tgcgggctac gagaaattcc tgaaggattc
      541 cgagaaacgt tattggttcg gttatgagga cctgggtcgt cgctccatgc acaaactgtg
      601 ggatctgatc aaagtcctgc cgaccttcgg catgatgcgt gctgatcgtt ccgtctacca
      661 gcacgcggcc ctgcgcgtca aggatgaacg cctgcgcatg gcgctgtcct tccatcctct
      721 gttcattggt ggtgatccgt tcaatgtgac cagcatgtac atcctggtat ctcaact
//
